Novel derivatives of mitomycin a,mitomycin b,and mitomycin c



May 26, 1970 MASZANAQ MAT$U| ET AL 3,514,452

NOVEL DERIVATIVES OF MITOMYCIN A, MITOMYCIN B, AND MITOMYCIIV C Filed June 5, 1964 16 Sheets-Sheet 1 WAVELENGTH 1) mcavr 24002000500 600 1/00 500 550 /400 wo 200 //00 MVE/VUME/Wcm") 4/ r2 INVENTORS ATTORNEYS May 26, 1970 MAS ANAO MATSUI ET 3,514,452

NOVEL DERIVATIVES OF MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN C Filed June 5, 1964 16 Sheets-Sheet :5

FIG. 5 WAVELENGTH 1) x 4000 3600 3200 2000 22100 2000 000 4900 0'00 000 500 M00 1300 mo //00 /000 900000 700 600 PERCENT .WNSMSS/O/V a B 8 6 $3 a $5 a g 8 8 WAVELENGTH 1) 50 .ZQ 8.

FIG. 6

l VZJVE/VU/l IBER/CM'? 64M. M/ I,

9 mfozzs May 26, 1970 MASANAO MATSUI ETAL 3,514,452

NOVEL DERIVATIVES 0F MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN G Filed June 5, 1964 16 Sheets-Sheet 4 INVENTOR R UR MayZG, 1970 MASANAO MATSUI ET AL 3,514,452

NOVEL DERIVATIVES OF MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN C 16 Sheets-Sheet 6 Filed June 5, 1964 FIG. /0 WAVELE/VGTHQU m E M m m m m Jm w 1 A B m w m m m u/ f A m L. m f m m Tm Y M m B E M WW m m W WM w w w m u. m m W F m .0. m x mm. w m w w w m \Q\.wu \m .QFNQNVWQ May 26, 1970 MASANAO MATSUI ETAL 3,514,452

NOVEL DERIVATIVES 0F MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN C Filed June 5, 1964 16 Sheets-Sheet 7 a0 /0 2 anus a IMlVELE/VGTH WAVELENGTH 1) 400036003200260024002000/9004500/700 600500140013? IZOD/I00 I000 900 600 700 0 ww mwwwwmm WAVHVWBER (607 I ENTORS BY7JMM 1 M ATTORNEYS May 26, 1970 MASANAO MATSUI ETAL NOVEL DERIVATIVES OF-MITOMYCIN A, MITOMYGIN B, AND MITOMYCIN 0 Filed June 5, 1964 16 Sheets-Sheet 8 Qmw QQN Qhk 8% QMQ QQQ QMQ 8Q QWQ QQQ p 8% QQQQQQ OGQQQQ Eng 8% 8% QQNN \T Q @MQQDEM: vi

Um Gk Sm BY 21:44 a Z flaw! ATTORNEYS May 26, 1970 MASANAO MATSUI ET AL 3,514,452

NOVEL DERIVATIVES OF MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN 0 Filed June 5, 1964 16 Sheets-Sheet 11 PERCENT TRANS/W/SS/Ofl/ mammawm FIG. 20 I WAVELENGTHQ/ .0 69 v 2 V .22 000W? Mu... .0 L l. 0 40003600 3200 2000 24002000 000 A900 000 $00 /500 /400 000 /200 000 1000 900 000 700 000% I I AI/HVUMBER (0/77") BY MW M ATTORNEYS May 26, 1970 MASANAQ su ET AL 3,514,452

NOVEL DERIVATIVES OF MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN c Filed June 5, 1964 16 Sheets-Sheet 12 PERCENT TEA/VSM/SS/O/V MER%%%%R%%% FIG. 23 WAVELENGTH 30 4.0 50 0 0 2 0 00 v00 /0 2 ug/ 55km PERCENT TRAIVSYl l/SS/O/Y q B '8 8 8 E 8 8 8 400036003200 290024002000500 4900 /700 I600 /500 /400 I300 /200 //00 I000 900 000 700 000 Wfll/HVUMBER (0/77"? Mam/00 W INVENTOR May 26, 1970 MASANAO MATSUl ETAL NOVEL DERIVATIVES 0F MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN C Filed June 5, 1964 16 Sheets-Sheet 15 Q & E

gmwgwm g 5 m A/O/SS/WS/Wil i/VJOHJd EfiiOR- law/ M $1M I,

May 26, 1970 MASANAO MATSUI ETAL 3,514,452

NOVEL DERIVATIVES 0F MITQMYCIN A, MITQMYCIN B, AND MITOMYCIN C Filed June 5, 1964 16 Sheets-Sheet 14 E. w w m 51 M 0 M n 6 Mmm w w m mm ZZZZ m m m m w w w w m m 4000x00m29o02a'0ozv'0o/9b0 A900 1700 Boob-'00 1400 4300 1200 1/60 K500 9'00 800' 760 BYu) y 1970 MASANAO MATSUI ET NOVEL DERIVATIVES 0F MITOMYCIN A, MITOMYCIN B, AND MITOMYCIN C Filed June 5, 1964 16 Sheets-Sheet l6 Kw wt QM BY WM 4 Z find/4 ATTORNEY! ABSTRACT OF THE DISCLOSURE A compound of the formula:

CHzO OONH:

wherein X is selected from the group consisting of OCH NH R NH,

and l 1 tr R being an alkyl group having from 1 to 7 carbon atoms, an aryl group, a cyclohexyl group or (CH OH wherein m is 2, R and R being an alkyl group having from 1 to 7 carbon atoms or an aryl group and R being (CH wherein n is an integer from 2 to 5, Y is selected from the group consisting of H and CH except that Y cannot be CH when X is NH and Z is H or CH and Z is selected from the group consisting of H, an alkyl group having from 1 to 4 carbon atoms, an alkanoyl group, a benzoyl group, a nuclear-chlorinated benzoyl group,

--o -0 H=C HQ The present invention relates to new antibiotics, moreparticularly to new derivatives of mitosane-compounds. In 1956, Hata et al. isolated new antibiotics from the United States Patent 0 fermented broth of Streplomyces Caespitosus and named the antibiotics Mitomycin A and B. In 1958, Wakaki et a1. isolated a third antibiotic from the same broth and named it Mitomycin C. Since then, studies on the determination of the above antibiotics have been made and recently, Webb et al. determined the chemical composition of the three compounds as follows:

Mitomycin A 3,514,452 Patented May 26, 1970 HaC Mitomycin C These compounds have the following skeleton:

O A 9 ornoooNrn 7 Etc-' 3 and are called mitosane-compounds.

As antibiotics, Mitomycin A, B and C have excellent efiect but also they have a defect i.e., they are poisonous to human blood. In order to obtain less poisonous mitosane-compounds, some researches had been conducted even before the determination of the chemical composition of Mitomycin A, B and C; for example, Monomethylmitomycin C or Porifiromycin was synthesized by reacting Mitomycin C and methyliodide in the presence of potassium carbonate. After the determination of the chemical composition of Mitomycin A, B and C, researches to obtain derivatives have been actively conducted. For example, Belgian Pat. 624,559 provides some 6,7 and la-substituted derivatives of mitosane-compounds.

The present inventors have conducted man researches to obtain derivatives of mitosane-compounds and have found several types of derivatives.

One object of the present invention is to provide new derivatives of mitosane-compounds, and another object is to provide the processes to produce the said derivatives. Further object of the present invention is to provide less poisonous derivatives of mitosane-compounds and other objects are clear from the following description.

The derivatives of mitosane-compounds of the present invention are represented by the following formula:

0 H CHzOCONH,

wherein X is or NR R and 'R are selected from the group consisting of H, alkyl-group and aryl-group, and R is (CH (n is an integral number), Y is H or CH and Z is selected from the group consisting of H, alkyl-group and alkanoyl-group, excluding the cases of X=NH Y=CH and Z=H, and X=NH Y=CH and Z:CH

According to the present invention, when an OCH group exists at the 7-position of mitosane, the group is replaced by an X group by reacting with XH, and when H exists at the la-position of mitosane, the said H is replaced by alkyl by reacting with a alkyl halide such as an alkyl chloride and an alkyl bromide; further, the above H may be replaced by an alkanoyl-group by reacting with aliphatic or aromatic carboxylic acid chloride or such acid anhydride. The OCH or OH group at the 9a-position is, however, not afiected by the above treatment. Therefore, in the present invention, when Y of the new derivatives is CH Mitomycin A or C is favorably used as the starting material while when Y is H, Mitomycin B is used as the starting material. Thus, the reactions in the present invention are generally classified to two types of reactions and more specifically classified to three. They are as follows:

(1) Replacement of OCH of 7-position by X by reacting with XH.

(i) Replacement of H of la-position by alkyl group by reacting with alkyl halide.

(ii) Replacement of H of la-position by alkanoyl group including benzoyl group by reacting With aliphatic or aromatic car-boxylic acid halide or such acid anhydride.

The above-mentioned reactions are the principal reactions in the present invention, and following are the illustrative explanations of the reactions and intermediates of the present invention somewhat more in detail.

(1) Mitomycin A is dissolved in a suitable solvent such as acetone. Alkyl halide and alkali metal carbinate are added to the solution and heated for several hours with reflux. Alkali metal carbonate is filtered oil? from the reaction mixture and the filtrate is concentrated by evaporation. The concentrate is adsorbed on silica gel packed in a column, and then developed and eluted by using ethyl acetate. The main fraction is concentrated, red colored crystals, being obtained. The product has the chemical composition of the formula:

H CH2 0 o 0 run (wherein Z is alkyl).

The above-mentioned reaction is shown by the following reaction scheme The compound I is dissolved in alcohol such as methanol and an excessive amount of amine (XH) is added. The mixture is allowed to stand for a While, whereupon the color of the mixture changes to bluish from red purple. The reaction mixture is then concentrated and if crystallization takes place, the crystals are filtered, and the filrtate is concentrated. The concentrate is adsorbed on silica packed in a column and developed and eluated by using as solvent acetone: ethyl acetate=1:1. The main fraction is evaporated. The crystallized product is separated. If crystallization does not take place, ether is added to the reaction mixture and the precipitate is separated. The reaction is shown by the following reaction scheme;

H ornoooNrn OCH (2) Mitomycin A is dissoived in a suitable solvent such as tetrahydrofuran, dioxane, benzene, ether, etc. Triethylamine and carboxylic acid chloride (*RCOCl) or carboxylic acid anhydride (RCO-O-OCH) are added to the solution and the reaction mixture is stirred, whereupon the hydrochloric acid salt of triethylamine is precipitated and filtered ofli. The filtrate is dried and the residue is dissolved in ethyl acetate and a chromatographtreat-ment is carried out as described in the first part of (1) supra. The main fraction is concentrated and crystallization is eifected. If necessary, either is added to crystallize the product. The reaction is represented by the reaction scheme;

01120 0 ONE: OCH; \I/\N o l NRCO (R is alkyl or aryl group.)

The compound III is treated as described in the latter III part of 1) and the reaction isrepresented by the reaction of (1) and the first part of (2) and is expressed by the v.1 reaction scheme;

scheme;

CHaOCONHa IOCHS +XH HaC I )mco p 0 m 5 I q l CHzOCONH:

CHgOCONHz I 0 CH3 x me 0am I N aC 0 NH N NRCO V CHzOCONH:

HaCQ i v 'OCHS H 'HsC "u t Mitomyoin A" (5) Mitomycin B is treated as described in the latter part of (1). The reaction is represented by the reaction 0 scheme; CH2OCONH2 4 00H: 1 H v N: J jn NEH.

l CHzOCONHa I l 0 0H, Z-halide H3O 1: 1 i

ii NH.- 1 .1 I

; cmoco N:

a Ha As is clear from the above description, la-posltion sub- N stitution can be carried out prior to 7-position substitution NZ and vice versa. Further, as is clear from the before-mentioned description, the compounds H, IV, and VI are new n derivatives of the present invention; moreover, the com pounds I, HI and V are also within the scope of the present invention, and they are not only important as the intermediates but also may have excellent antibiotic activity. Y j The following examples are only for more specifically (Z is alkyl). I illustrating the present invention but are not intended to be (,4)- The process is the combination of the latter part limitative of the present invention.

N g I NCHs To 250 mg. of Mitomycin A dissolved in 10 ml. of acetone was added 500 mg. of anhydrous potassium carbonate and 1 ml. of methyl iodide. The mixture was refiuxed with stirring for 3 hours. The reaction mixture was filtered to remove potassium carbonate and the filtrate was evaporated under reduced pressure to dryness. The red colored residue was dissolved in 5 m1. of ethyl acetate and the solution was introduced onto a column 1.5 x20 cm.) of silicic acid. Development with ethyl acetate gave two bands of red color. The first major band contained the reaction product and the second minor band contained the original Mitomycin A. The first band was eluted and the eluate was evaporated to dryness and the residue was crystallized from ether. 150 mg. of the red colored methyl Mitomycin A was obtained. MJP. 165 C.

Analysis.Calcd for C H O N (percent); C, 56.19; H, 5.83; N, 11.57. Found (percent): C, 56.05; H, 5.70; N, 1 1.40.

The infrared spectrum in Nujol is shown in FIG. 1.

EXAMPLE 2 H360 01120 c ONHz I OCH; H3O

To 50 mg. of la-methyl Mitomycin A dissolved in ml. of methanol was introduced n-propylamine. After standing for 20 minutes at room temperature, the color of the solution was changed to bluish purple from reddish purple. The reaction mixture was evaporated under reduced pressure to drynes. The residue was chromatographed on silicic acid using ethyl acetate as the solvent system. The bluish band was developed and eluted. The eluate was evaporated under reduced pressure. The residue was crystallized from ethyl acetate-ether. 40 mg. of purple needles was obtained. M.P. 178 C.

Analysis.-Calcd for C H O N (percent): C, 58.45; H, 6.71; N, 14.35. Found (percent): C, 58.20; 6.50; N,

The infra-red spectrum thereof in Nujol is shown in FIG. 2.

EXAMPLE 3 la-methyl 7a-ethyl Mitomycin C was prepared by the same procedure as Example 2. Purple needles. M.P. 170 C.

8 Analysis.Calcd for C H O N (percent): C, 57.43; H, 6.71; N, 14.35. Found (percent): C, 58.20; H, 6.50; N, 14.98.

The infrared spectrum in Nujol is shown in FIG. 3.

5 EXAMPLE 4 o omo 0 ONE:

l O CH3 11.0

o0o1 i N 0 NH CHzOCONHz JV oom HO a \N t) L N00 500 mg. of Mitomycin A and 1 ml. of triethylamine were dissolved in 20 ml. of anhydrous tetrahydrofuran. A sOlution of 190 mg. of benzoyl chloride in 3 ml. of tetrahydrofuran was introduced dropwise with stirring at room temperature. When the mixture was stirred for 15 minutes, crystalline triethylamine hydrochloride was precipitated gradually. The reaction mixture was filtered to remove triethylamine hydrochloride and the filtrate Was evaporated to dryness. The residue was dissolved in ethyl acetate (5 ml.) and chromatographed on silicic acid (2X cm.). Development with ethyl acetate gave two bands. The first major band was eluted and the eluate was evaporated to dryness. The residue was crystallized from ether. 430 mg. of purple needles was obtained. M.P. 158 C.

Analysis.-Calcd for C H O N (percent): C, 60.92; H, 5.11; N, 9.27. Found (percent): C, 60.49; H, 5.01; N, 9.37.

The infra-red spectrum is shown in FIG. 4.

EXAMPLE 5 la-caproyl Mitomycin A was prepared by the same procedure as Example 4. Red needles. M.P. 98 C.

Analysis.--Calcd for C 'H O N (percent): C, 59.05; $1256.53; N, 9.39. Found (percent): C, 58.75; H, 6.45; N,

The infra-red spectrum in Nujol is shown in FIG. 5.

EXAMPLE 6 0 H2O C ONHz OCH3 I I1. C3H7NH2 CHQO 0 ONE:

port, 

